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online neb lamp primer design tool  (New England Biolabs)


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    Structured Review

    New England Biolabs online neb lamp primer design tool
    The <t> LAMP primer </t> used in the present study.
    Online Neb Lamp Primer Design Tool, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/online+neb+lamp+primer+design+tool/Control+LAMP+Primer+Mix/pmc11695371-139-19-20
    Average 95 stars, based on 98 article reviews
    online neb lamp primer design tool - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method"

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2024.1485884

    The  LAMP primer  used in the present study.
    Figure Legend Snippet: The LAMP primer used in the present study.

    Techniques Used: Sequencing

    Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.
    Figure Legend Snippet: Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

    Techniques Used: Selection, CRISPR

    Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.
    Figure Legend Snippet: Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

    Techniques Used: Infection, Amplification, CRISPR, Recombinant, Plasmid Preparation, Positive Control

    Related Articles

    Sequencing:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    Selection:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    CRISPR:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    Infection:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    Amplification:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    Recombinant:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    Plasmid Preparation:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T

    Positive Control:

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ). .. T



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    New England Biolabs online neb lamp primer design tool
    The <t> LAMP primer </t> used in the present study.
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    Image Search Results


    The  LAMP primer  used in the present study.

    Journal: Frontiers in Plant Science

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    doi: 10.3389/fpls.2024.1485884

    Figure Lengend Snippet: The LAMP primer used in the present study.

    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

    Techniques: Sequencing

    Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

    Journal: Frontiers in Plant Science

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    doi: 10.3389/fpls.2024.1485884

    Figure Lengend Snippet: Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

    Techniques: Selection, CRISPR

    Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

    Journal: Frontiers in Plant Science

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    doi: 10.3389/fpls.2024.1485884

    Figure Lengend Snippet: Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

    Techniques: Infection, Amplification, CRISPR, Recombinant, Plasmid Preparation, Positive Control

    The  LAMP primer  used in the present study

    Journal: Microbiology Spectrum

    Article Title: One-tube detection of Salmonella Typhimurium using LAMP and CRISPR-Cas12b

    doi: 10.1128/spectrum.01271-24

    Figure Lengend Snippet: The LAMP primer used in the present study

    Article Snippet: The Online NEB LAMP primer design tool ( https://lamp.neb.com/ #!/) was used to design the LAMP primer set targeting the ST gene STM4497 (Gene ID: 1256023).

    Techniques: Sequencing