online neb lamp primer design tool (New England Biolabs)
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Online Neb Lamp Primer Design Tool, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/online+neb+lamp+primer+design+tool/Control+LAMP+Primer+Mix/pmc11695371-139-19-20
Average 95 stars, based on 98 article reviews
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1) Product Images from "Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method"
Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
Journal: Frontiers in Plant Science
doi: 10.3389/fpls.2024.1485884
Figure Legend Snippet: The LAMP primer used in the present study.
Techniques Used: Sequencing
Figure Legend Snippet: Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.
Techniques Used: Selection, CRISPR
Figure Legend Snippet: Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.
Techniques Used: Infection, Amplification, CRISPR, Recombinant, Plasmid Preparation, Positive Control
Related Articles
Sequencing:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Selection:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the CRISPR:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Infection:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Amplification:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Recombinant:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Plasmid Preparation:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Positive Control:Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the |
